Chrysin possesses potent anti-inflammation and anti-oxidation properties. This nutrient is able to stimulate nitric oxide release from endothelial cells leading to vascular cGMP accumulation and subsequent endothelium dependent vascular relaxation. Chrysin stimulated nitric oxide release is calcium independent and possibly mediated via PI3-kinase. Chrysin is able to suppress liposacharide-induced COX-2 protein and mRNA expression in a dose-dependent manner. Nuclear factor for IL-6 has been identified as responsible for the chrysin -mediated COX-2 downregulation. There are claims that chrysin stimulates the release of testosterone. Since chrysin is available as an herbal supplement, some users, for instance body builders, are taking chrysin with the hope of raising testosterone levels or stimulating testosterone production. One study listed below did not find chrysin supplementation leads to any significant increase in testosterone production.